期刊：
Nanoscale Research Letters,2008年3(11):397-415 ISSN：1931-7573
通讯作者：
Jiang, C.(czjiang@whu.edu.cn)
作者机构：
[Quanguo He] Key Laboratory of Green Packaging and Bio-Nanotechnology Applications (Hunan Province), Hunan University of Technology, Zhuzhou 412008, China;[Changzhong Jiang; Wei Wu] Department of Physics, Wuhan University, Wuhan 430072, China
通讯机构：
[Wu, Wei] Department of Physics, Wuhan University, Wuhan, 430072, People's Republic of China.
关键词：
Bio-molecules - Functionalized - Future trends - Inorganic materials - Interactive functions - Magnetic iron oxide nanoparticles - Magnetic iron oxide NPs - Magnetic saturations - Novel functional materials - Organic materials - Practical - Preparation - Recent developments - Research areas - Surface functionalization
摘要：
Surface functionalized magnetic iron oxide nanoparticles (NPs) are a kind of novel functional materials, which have been widely used in the biotechnology and catalysis. This review focuses on the recent development and various strategies in preparation, structure, and magnetic properties of naked and surface functionalized iron oxide NPs and their corresponding application briefly. In order to implement the practical application, the particles must have combined properties of high magnetic saturation, stability, biocompatibility, and interactive functions at the surface. Moreover, the surface of iron oxide NPs could be modified by organic materials or inorganic materials, such as polymers, biomolecules, silica, metals, etc. The problems and major challenges, along with the directions for the synthesis and surface functionalization of iron oxide NPs, are considered. Finally, some future trends and prospective in these research areas are also discussed.
摘要：
Graphene exhibits unique 2-D structure and exceptional phyiscal and chemical properties that lead to many potential applications. Among various applications, biomedical applications of graphene have attracted ever-increasing interests over the last three years. In this review, we present an overview of current advances in applications of graphene in biomedicine with focus on drug delivery, cancer therapy and biological imaging, together with a brief discussion on the challenges and perspectives for future research in this field.
摘要：
Gene transfer methods are promising in the field of gene therapy. Current methods for gene transfer include three major groups: viral, physical and chemical methods. This review mainly summarizes development of several types of chemical methods for gene transfer in vitro and in vivo by means of nano-carriers like; calcium phosphates, lipids, and cationic polymers including chitosan, polyethylenimine, polyamidoamine dendrimers, and poly(lactide-co-glycolide). This review also briefly introduces applications of these chemical methods for gene delivery.
期刊：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2013年9(8):1378-1382 ISSN：1550-7033
通讯作者：
Deng, Y.(dy_dengyan@yahoo.com.cn)
作者机构：
[Li, Zhiyang; Ma, Chao; Deng, Yan] State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China;[Wang, Wei; Deng, Yan] Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China
通讯机构：
[Deng, Yan] Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China.
期刊：
Chemistry of Materials,2014年26(5):1794-1798 ISSN：0897-4756
通讯作者：
He, NY
作者机构：
[Deng, Yan; Xia, Kai; Li, Song; He, Nongyue; Zhang, Liming; Lu, Zhuoxuan] Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412008, China;[Zhou, Feimeng] Department of Chemistry and Biochemistry, California State University, Los Angeles, CA 90032, United States;[He, Nongyue; Li, Guopeng; Zhang, Liming; Lu, Zhuoxuan; Chen, Juan] State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
通讯机构：
[He, Nongyue] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412008, Peoples R China.
作者机构：
[Li, Song; Zhang, Liming; Lu, Zhuoxuan; Deng, Yan; He, Nongyue] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412007, Peoples R China.;[Li, Song; Zhang, Liming; Lu, Zhuoxuan; Deng, Yan; Jia, Yingying; He, Nongyue; Liu, Hongna] Southeast Univ, State Key Lab Bioelect, Dept Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China.;[He, Nongyue] Southeast Univ, State Key Lab Bioelect, Dept Biol Sci & Med Engn, Sipailou 2, Nanjing 210096, Jiangsu, Peoples R China.
通讯机构：
[He, Nongyue] Southeast Univ, State Key Lab Bioelect, Dept Biol Sci & Med Engn, Sipailou 2, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
Magnetic beads;Dual-color SBE;Single nucleotide polymorphisms;Gold magnetic nanoparticles;Microarray
摘要：
To fulfill the increasing need for large-scale genetic research, a high-throughput and automated SNPs genotyping method based on gold magnetic nanoparticles (GMNPs) array and dual-color single base extension has been designed. After amplification of DNA templates, biotinylated extension primers were captured by streptavidin coated gold magnetic nanoparticle (SA-GMNPs). Next a solid-phase, dual-color single base extension (SBE) reaction with the specific biotinylated primer was performed directly on the surface of the GMNPs. Finally, a "bead array" was fabricated by spotting GMNPs with fluorophore on a clean glass slide, and the genotype of each sample was discriminated by scanning the "bead array". MTHFR gene C677T polymorphism of 320 individual samples were interrogated using this method, the signal/noise ratio for homozygous samples were over 12.33, while the signal/noise ratio for heterozygous samples was near 1. Compared with other dual-color hybridization based genotyping methods, the method described here gives a higher signal/noise ratio and SNP loci can be identified with a high level of confidence. This assay has the advantage of eliminating the need for background subtraction and direct analysis of the fluorescence values of the GMNPs to determine their genotypes without the necessary procedures for purification and complex reduction of PCR products. The application of this strategy to large-scale SNP studies simplifies the process, and reduces the labor required to produce highly sensitive results while improving the potential for automation.
期刊：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2013年9(4):736-740 ISSN：1550-7033
通讯作者：
Deng, Y
作者机构：
[Lin, Lin; Deng, Yan; Li, Song; Xu, Lijian; Wang, Wei; Lu, Zhuoxuan; Zhang, Liming] Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China;[Liu, Hongna; Deng, Yan] State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
通讯机构：
[Deng, Yan] Hunan Univ Technol, Key Lab Green Packaging & Applicat Biol Nanotechn, Zhuzhou 412007, Peoples R China.
期刊：
Biosensors and Bioelectronics,2010年26(4):1442-1448 ISSN：0956-5663
通讯作者：
He, N.(nongyue580210@yahoo.com.cn)
作者机构：
[Tian, Lan; Li, Song; He, Nongyue] Key Lab. of Green Packaging and Application of Biological Nanotechnology of Hunan Province, Hunan University of Technology, Zhuzhou 412008, China;[Li, Song; He, Nongyue; Liu, Hongna; Liu, Lishang] State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
通讯机构：
[He, Nongyue] Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Peoples R China.
会议名称：
World Congress on Biosensors
会议时间：
MAY 26-28, 2010
会议地点：
Scotland, ENGLAND
会议主办单位：
[Liu, Hongna;Li, Song;Tian, Lan;He, Nongyue] Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Peoples R China.^[Li, Song;Liu, Lishang;He, Nongyue] Hunan Univ Technol, Key Lab Green Packaging & Applicat Biol Nanotechn, Zhuzhou 412008, Peoples R China.
摘要：
A sensitive and selective biosensor platform suited for SNP type using Fe@Au magnetic nanoparticles (GMNPs) to fabricate bead array is described. This new platform integrates the rapid binding kinetics of magnetic nanoparticles carriers, the multiplexing and encoding capabilities of chips, and tagged array. As a DNA sensor, the biotinylated single-stranded DNA was obtained by asymmetry PCR amplification, and then captured by GMNPs modified with streptavidin to form GMNP-ssDNA complexes without further purification. The complexes were immobilized on the slide to fabricate bead array through magnetic field. The bead array was hybridized with the corresponding allele-specific tag probes for each locus, and a pair of given universal detectors were applied to these markers analysis. Using bead array, all samples can be analyzed in one hybridization chamber which lowers the cost of the assay. Using universal tags, only a pair of universal dual-color probes labeled fluorophores was used for multiplex genotyping. Without the need of laborious and time-consuming elution, the experiment process was simple, reproducible and easy to handle. Two SNPs loci from 12 individual samples were discriminated using this platform and the results demonstrated that the expected scores and good discrimination were obtained between the two alleles from the two SNP loci. In summary, the integrated sensitive platform is adaptable and versatile, while offering a high-throughput capability needed for genome research and clinical applications. (C) 2010 Elsevier B.V. All rights reserved.
作者：
Ji, Xuyuan;Yang, Wenjing;Wang, Ting;Mao, Chun;Guo, Lingling;Xiao, Jiangqiang;He, Nongyue*
期刊：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2013年9(10):1672-1678 ISSN：1550-7033
通讯作者：
He, Nongyue
作者机构：
[Ji, Xuyuan; Guo, Lingling; He, Nongyue; Wang, Ting] State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Nanjing, China;[Xiao, Jiangqiang] Department of Hepatobiliary Surgery, Nanjing University Medical School, Nanjing, China;[He, Nongyue] Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, China;[Yang, Wenjing] School of Medical Science and Laboratory Medicine, Jiangsu University, Zhenjiang, China;[Mao, Chun] School of Chemistry and Materials Science, Nanjing Normal University, Nanjing, China
通讯机构：
[He, Nongyue] Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.
期刊：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2015年15(1):54-62 ISSN：1533-4880
通讯作者：
He, Nongyue
作者机构：
[Mou, Xianbo; Li, Song; He, Nongyue; Ali, Zeeshan] State Key Laboratory of Bioelectronics, Southeast University, Nanjing, China;[Li, Song; He, Nongyue] Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, China
通讯机构：
[He, Nongyue] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
Drug delivery system - Magnetic nano-particles - Magnetic nanoparti cles (MNPs) - Superparamagnetic property - Surface Functionalization - Targeted delivery systems - Targeted drug delivery systems - Temperature sensitivity
摘要：
Magnetic nanoparticles (MNPs) are a special kind of nanomaterials and widely used in biomedical technology applications. Currently they are popularly customized for disease detection and treatment, particularly as drug carriers in drug targeted delivery systems, as a therapeutic in hyperthermia (treating tumors with heat), and as contrast agents in magnetic resonance imaging (MRI). Due to their biocompatibility and superparamagnetic properties, MNPs as next generation drug carriers have great attraction. Although the potential benefits of MNPs are considerable, any potential toxicity associated with these MNPs should be identified distinctly. The drug loading capability and the biomedical properties of MNPs generated by different surface coatings are the most sensitive parameters in toxicity. A lot of organic and inorganic materials are utilized as coating materials for surface functionalization and reducing toxicity of MNPs. pH or temperature sensitivity materials are widely used to manage drug loading and targeted release. In addition, MNPs can be controlled and directed to the desired pathological region by using external magnetic files (EMF). The realization of targeted drug delivery has decreased the dosage and improved the efficiency of drugs, which results in reduced side effects to normal tissues. This review discussed the possible organ toxicities of MNPs and their current advances as a drug delivery vehicle.
期刊：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2013年9(4):674-684 ISSN：1550-7033
通讯作者：
He, N.(nyhe1958@163.com)
作者机构：
[Xi, Zhijiang; Jin, Lian; Liu, Ming; Deng, Yan; Li, Chuanyan; Jiang, Hongrong; He, Nongyue; Li, Zhiyang] State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;[Zeng, Xin] State Key Laboratory of Reproductive Medicine, Department of Gynecological Endocrine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China;[He, Nongyue; Deng, Yan] Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China;[Wang, Zhifei] School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
通讯机构：
[He, Nongyue] Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
Chemical reduction - D. transmission electron microscopes (TEM) - Energy dispersive spectrometers - Fourier transform infra red (FTIR) spectroscopy - Magnetic nanocomposites - Scanning electronic microscopes - Seed mediated - Self-assembling
摘要：
A method for highly sensitive and rapid detection of Pseudomonas aeruginosa, based on magnetic enrichment and magnetic separation, is described in this paper. The magnetic nanoparticles (MNPs) were applied to adsorb genome DNA after the sample was lysed. The DNA binding MNPs were directly subjected to polymerase chain reaction (PCR) to amplify gyrB specific sequence of Pseudomonas aeruginosa. The biotin labeled PCR products were detected by chemiluminescence when they were successively incubated with the probes-modified MNPs and alkaline phosphatase (ALP) labeled streptavidin (SA). Agarose gel electrophoresis analyses approved the method of in situ PCR to be highly reliable. The factors which could affect the chemiluminiscence were studied in detail. The results showed that the MNPs of 400 nm in diameter are beneficial to the detection. The sequence length and the binding site of the probe with a target sequence have obvious effects on the detection. The optimal concentration of the probes, hybridization temperature and hybridization time were 10 muM, 60 masculineC and 60 mins, respectively. The method of in situ PCR based on MNPs can greatly improve the utilization rate of the DNA template ultimately enhancing the detection sensitivity. Experiment results proved that the primer and probe had high specificity, and Pseudomonas aeruginosa was successfully detected with detection limits as low as 10 cfu/mL by this method, while the detection of a single Pseudomonas aeruginosa can also be achieved.
作者：
Xi, Zhijiang;Huang, Rongrong;Li, Zhiyang;He, Nongyue*;Wang, Ting;Su, Enben;Deng, Yan
期刊：
ACS Applied Materials and Interfaces,2015年7(21):11215-11223 ISSN：1944-8244
通讯作者：
He, Nongyue
作者机构：
[Xi, Zhijiang] School of Life and Science, Yangtze University, Jingzhou, China;[He, Nongyue; Su, Enben; Deng, Yan; Xi, Zhijiang; Li, Zhiyang; Wang, Ting; Huang, Rongrong] State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China;[He, Nongyue; Deng, Yan] Economical Forest Cultiv. and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, China;[Su, Enben] Getein Biotechnology Co., Ltd., Nanjing, China
通讯机构：
[He, Nongyue] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
aptamers;selection;SELEX;HBsAg;carboxylated magnetic nanoparticles;detection
作者机构：
[Jin, Lian; Li, Song; Liu, Ming; Elingarami, Sauli; Deng, Yan; Zhang, Gen; Liu, Hongna; Fan, Jing; Zeng, Xin; Yang, Haowen; He, Nongyue] 1. The State Key Laboratory of Bioelectronics, Department of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China;[Zeng, Yu; Zeng, Xin; Hu, Ping] 2. Nanjing Maternity and Child Health Care Hospital, Nanjing 210029, China;[Li, Song; Deng, Yan] 4. Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, P. R. China;[Zhang, Gen] 3. Nanjing Medical University, Nanjing 210029, China
通讯机构：
[Zeng, Xin] Southeast Univ, Dept Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
Copy number variation;Ligation-dependent PCR;Magnetic nanoparticles;Chemiluminescence
摘要：
A novel system for copy number variation (CNV) analysis was developed in the present study using a combination of magnetic separation and chemiluminescence (CL) detection technique. The amino-modified probes were firstly immobilized onto carboxylated magnetic nanoparticles (MNPs) and then hybridized with biotin-dUTP products, followed by amplification with ligation-dependent polymerase chain reaction (PCR). After streptavidin-modified alkaline phosphatase (STV-AP) bonding and magnetic separation, the CL signals were then detected. Results showed that the quantification of PCR products could be reflected by CL signal values. Under optimum conditions, the CL system was characterized for quantitative analysis and the CL intensity exhibited a linear correlation with logarithm of the target concentration. To validate the methodology, copy numbers of six genes from the human genome were detected. To compare the detection accuracy, multiplex ligation-dependent probe amplification (MLPA) and MNPs-CL detection were performed. Overall, there were two discrepancies by MLPA analysis, while only one by MNPs-CL detection. This research demonstrated that the novel MNPs-CL system is a useful analytical tool which shows simple, sensitive, and specific characters which are suitable for CNV analysis. Moreover, this system should be improved further and its application in the genome variation detection of various diseases is currently under further investigation.
作者：
Jiang, Hongrong;Zeng, Xin;He, Nongyue*;Deng, Yan;Lu, Guangming;Li, Kai
期刊：
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY,2013年13(3):1617-1625 ISSN：1533-4880
通讯作者：
He, Nongyue
作者机构：
[Li, Kai] Laboratory of Molecular Medicine, College of Pharmaceutical Science, Soochow University, Suzhou 215123, China;[Lu, Guangming] Department of Radiology, Nanjing University, Jin Ling Hospital of Nanjing, Nanjing 210002, China;[He, Nongyue; Deng, Yan; Jiang, Hongrong] State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China;[Zeng, Xin] State Key Laboratory of Reproductive Medicine, Department of Gynecological Endocrine, Nanjing Maternity, Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China;[Deng, Yan] Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China
通讯机构：
[He, Nongyue] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
Approaches - Biomedical applications - Biomedical fields - Magnetic nanocomposites - Physical and chemical properties - Preparation - Seed-mediated growth methods - Sonochemical synthesis
期刊：
NANOSCIENCE AND NANOTECHNOLOGY LETTERS,2016年8(9):785-790 ISSN：1941-4900
通讯作者：
Deng, Y
作者机构：
[Wang, Zunliang; Ma, Chao; Deng, Yan] State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China;[Ma, Chao] School of Medicine Information, Xuzhou Medical University, Xuzhou, 221004, China;[Dong, Hongming; Deng, Yan; Liu, Yuan; Liu, Keke; Zhang, Liming] Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green Chemistry and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, 412007, China
作者机构：
[Li, Xiaolong; Zhang, Fengqin; Deng, Yan; He, Nongyue; Zhang, Liming; Lu, Zhuoxuan] Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412008, China;[Li, Xiaolong; Ma, Chao; He, Nongyue; Zhang, Liming; Lu, Zhuoxuan] State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
通讯机构：
[Lu, Zhuoxuan] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412008, Peoples R China.
作者机构：
[Li, Song; Liu, Hongna; He, Nongyue; Tian, Lan] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Peoples R China.;[Li, Song; Liu, Lishang; He, Nongyue] Hunan Univ Technol, Key Lab Green Packaging & Applicat Biol Nanotechn, Zhuzhou 412008, Peoples R China.
通讯机构：
[He, Nongyue] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Peoples R China.
关键词：
Gold magnetic nanoparticle array;Dual-color hybridization;Single-nucleotide polymorphisms
摘要：
A high-throughput and cost-effective single-nucleotide polymorphism (SNP) genotyping method based on a gold magnetic nanoparticle (GMNP) array with dual-color hybridization has been designed. Biotinylated single-strand polymerase chain reaction (PCR) products containing the SNP locus were captured by the GMNPs that were coated with streptavidin. The GMNP array was fabricated by immobilizing single-stranded DNA (ssDNA)-GMNP complexes onto a glass slide using a magnetic field, and SNPs were identified with dual-color fluorescence hybridization. Three different SNP loci from 24 samples were genotyped successfully using this platform. This procedure allows the user to directly analyze the bead fluorescence to determine the SNP genotype, and it eliminates the need for background subtraction for signal determination. This method also bypasses tedious PCR purification and concentration procedures, and it facilitates large-scale SNP studies by using a method that is highly sensitive, simple, labor-saving, and potentially automatable. (C) 2010 Elsevier Inc. All rights reserved.
