摘要：
Graphene exhibits unique 2-D structure and exceptional phyiscal and chemical properties that lead to many potential applications. Among various applications, biomedical applications of graphene have attracted ever-increasing interests over the last three years. In this review, we present an overview of current advances in applications of graphene in biomedicine with focus on drug delivery, cancer therapy and biological imaging, together with a brief discussion on the challenges and perspectives for future research in this field.
关键词：
Chemical Methods.;Gene delivery;Non-viral;Vectors
摘要：
Gene transfer methods are promising in the field of gene therapy. Current methods for gene transfer include three major groups: viral, physical and chemical methods. This review mainly summarizes development of several types of chemical methods for gene transfer in vitro and in vivo by means of nano-carriers like; calcium phosphates, lipids, and cationic polymers including chitosan, polyethylenimine, polyamidoamine dendrimers, and poly(lactide-co-glycolide). This review also briefly introduces applications of these chemical methods for gene delivery.
期刊：
CHEMISTRY OF MATERIALS,2014年26(5):1794–1798 ISSN：0897-4756
通讯作者：
He, Nongyue
作者机构：
[Li, Song; Zhang, Liming; Lu, Zhuoxuan; Xia, Kai; Deng, Yan; He, Nongyue] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412008, Peoples R China.;[Li, Guopeng; Zhang, Liming; Lu, Zhuoxuan; He, Nongyue; Chen, Juan] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.;[Zhou, Feimeng] Calif State Univ Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90032 USA.
通讯机构：
[He, Nongyue] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412008, Peoples R China.
摘要：
Although gold nanorods (GNRs) have been prepared with a wide range of methods for their uses as novel diagnostic and therapeutic agents, the synthesis of monodispersed GNRs with high yields and size tunability still requires further improvements. We report on a simple one-pot method for preparing highly monodispersed GNRs using phenols (e.g., hydroquinone, 1,2,3-trihydroxybenzene, and 1,2,4-trihydroxybenzene) as the reducing agent and NaBH4 as the initiating reactant. Fine-tuning of the LSPR peak position of phenols-reduced GNRs from 550 to 1150 nm is accomplished by regulating the silver ion concentrations. The size of GNRs produced via phenols reduction can also be controlled by changing the NaBH4 concentration. By systematically optimizing the concentrations of the reagents involved in the one-pot synthesis of GNRs, the yield (in many cases exceeding 90%) is significantly higher than that prepared with the commonly used reductant (e.g., ascorbic acid). The improved efficiency and controllability cut down the cost and time involved in GNRs production.
摘要：
Using two nano-porous pseudo carbon paste electrodes (Nano-PPCPEs) as the working electrodes, a saturated calomel electrode as the reference electrode, and a Pt electrode as the counter electrode to form a four-electrode system, a novel type of glutamate-acetylcholine electrochemical biosensor array was successfully fabricated. It was shown that the biosensor array displayed a high selectivity and sensitivity and the selective and simultaneous determination of glutamate and acetylcholine (ATCh) is feasible. For L-glutamate, a linear range from 5 x 10(-7) M to 1 x 10(-5) M with the detection limit of 2.5 x 10(-7) M was demonstrated, while for ATChl the linear range is from 5 x 10(-6) M to 2 x 10(-4) M with the detection limit of 1.5 x 10(-7) M. This research means a possibility to simultaneously detect more neurotransmitters with a nano-PPCPEs electrochemical biosensor array.
期刊：
Journal of Biomedical Nanotechnology,2013年9(4):736-740 ISSN：1550-7033
通讯作者：
Deng, Y.(dy_dengyan@yahoo.com.cn)
作者机构：
[Lin, Lin; Deng, Yan; Li, Song; Xu, Lijian; Wang, Wei; Lu, Zhuoxuan; Zhang, Liming] Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China;[Liu, Hongna; Deng, Yan] State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
通讯机构：
[Deng, Yan] Hunan Univ Technol, Key Lab Green Packaging & Applicat Biol Nanotechn, Zhuzhou 412007, Peoples R China.
作者机构：
[Li, Song; Zhang, Liming; Lu, Zhuoxuan; Deng, Yan; He, Nongyue] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412007, Peoples R China.;[Li, Song; Zhang, Liming; Lu, Zhuoxuan; Deng, Yan; Jia, Yingying; He, Nongyue; Liu, Hongna] Southeast Univ, State Key Lab Bioelect, Dept Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China.;[He, Nongyue] Southeast Univ, State Key Lab Bioelect, Dept Biol Sci & Med Engn, Sipailou 2, Nanjing 210096, Jiangsu, Peoples R China.
通讯机构：
[He, Nongyue] Southeast Univ, State Key Lab Bioelect, Dept Biol Sci & Med Engn, Sipailou 2, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
Dual-color SBE;Gold magnetic nanoparticles;Magnetic beads;Microarray;Single nucleotide polymorphisms
摘要：
To fulfill the increasing need for large-scale genetic research, a high-throughput and automated SNPs genotyping method based on gold magnetic nanoparticles (GMNPs) array and dual-color single base extension has been designed. After amplification of DNA templates, biotinylated extension primers were captured by streptavidin coated gold magnetic nanoparticle (SA-GMNPs). Next a solid-phase, dual-color single base extension (SBE) reaction with the specific biotinylated primer was performed directly on the surface of the GMNPs. Finally, a "bead array" was fabricated by spotting GMNPs with fluorophore on a clean glass slide, and the genotype of each sample was discriminated by scanning the "bead array". MTHFR gene C677T polymorphism of 320 individual samples were interrogated using this method, the signal/noise ratio for homozygous samples were over 12.33, while the signal/noise ratio for heterozygous samples was near 1. Compared with other dual-color hybridization based genotyping methods, the method described here gives a higher signal/noise ratio and SNP loci can be identified with a high level of confidence. This assay has the advantage of eliminating the need for background subtraction and direct analysis of the fluorescence values of the GMNPs to determine their genotypes without the necessary procedures for purification and complex reduction of PCR products. The application of this strategy to large-scale SNP studies simplifies the process, and reduces the labor required to produce highly sensitive results while improving the potential for automation.
作者机构：
[Wang, Ting; Xi, Zhijiang; Huang, Rongrong; Deng, Yan; Su, Enben; Li, Zhiyang; He, Nongyue] daggerState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, P. R. China;[Xi, Zhijiang] double daggerSchool of Life and Science, Yangtze University, Jingzhou 434025, P. R. China;[Deng, Yan; He, Nongyue] section signEconomical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, P. R. China;[Su, Enben] parallelGetein Biotechnology Co., Ltd., Nanjing 210000, P. R. China
通讯机构：
[He, Nongyue] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
HBsAg;SELEX;aptamers;carboxylated magnetic nanoparticles;detection;selection
摘要：
Aptamers are short single-stranded DNA or RNA oligonucleotides and can be selected from synthetic combinatorial libraries in vitro. They have a high binding affinity and specificity for their targets. Agarose gels, nitrocellulose membranes, and adsorptive microplates are often used as carriers to immobilize targets in the SELEX (systematic evolution of ligands by exponential enrichment) process, but the subsequent separation step is tedious and time-consuming. Therefore, we used magnetic nanoparticles (MNPs) as carriers to immobilize the target, hepatitis B surface antigen (HBsAg), which is convenient for fast magnetic separation. In this study, we first selected DNA aptamers against HBsAg by immobilizing HBsAg on the surface of carboxylated MNPs. The ssDNA library of each selection round was prepared by asymmetric PCR amplification for the next selection round. To obtain aptamer sequences, the final selected products were purified by gel electrophoresis, then cloned, and sequenced. DNA aptamers that specifically bind to HBsAg were successfully obtained after 13 selection rounds. The selected aptamers were used to construct a chemiluminescence aptasensor based on magnetic separation and immunoassay to detect HBsAg from pure protein or actual serum samples. There was a linear relationship between HBsAg concentration and chemiluminescent intensity in the range of 1-200 ng/mL. The aptasensor worked well even in the presence of interfering substances and was highly specific in the detection of HBsAg in serum samples, with a detection limit 0.1 ng/mL lower than the 0.5 ng/mL limit of an ELISA in use at the hospital. This aptasensor can contribute to better detection of hepatitis B virus infection.
通讯机构：
[He, Nongyue] Southeast Univ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
Magnetic Nanoparticle;Toxicity;Drug Delivery System
摘要：
Magnetic nanoparticles (MNPs) are a special kind of nanomaterials and widely used in biomedical technology applications. Currently they are popularly customized for disease detection and treatment, particularly as drug carriers in drug targeted delivery systems, as a therapeutic in hyperthermia (treating tumors with heat), and as contrast agents in magnetic resonance imaging (MRI). Due to their biocompatibility and superparamagnetic properties, MNPs as next generation drug carriers have great attraction. Although the potential benefits of MNPs are considerable, any potential toxicity associated with these MNPs should be identified distinctly. The drug loading capability and the biomedical properties of MNPs generated by different surface coatings are the most sensitive parameters in toxicity. A lot of organic and inorganic materials are utilized as coating materials for surface functionalization and reducing toxicity of MNPs. pH or temperature sensitivity materials are widely used to manage drug loading and targeted release. In addition, MNPs can be controlled and directed to the desired pathological region by using external magnetic files (EMF). The realization of targeted drug delivery has decreased the dosage and improved the efficiency of drugs, which results in reduced side effects to normal tissues. This review discussed the possible organ toxicities of MNPs and their current advances as a drug delivery vehicle.
摘要：
A method for highly sensitive and rapid detection of Pseudomonas aeruginosa, based on magnetic enrichment and magnetic separation, is described in this paper. The magnetic nanoparticles (MNPs) were applied to adsorb genome DNA after the sample was lysed. The DNA binding MNPs were directly subjected to polymerase chain reaction (PCR) to amplify gyrB specific sequence of Pseudomonas aeruginosa. The biotin labeled PCR products were detected by chemiluminescence when they were successively incubated with the probes-modified MNPs and alkaline phosphatase (ALP) labeled streptavidin (SA). Agarose gel electrophoresis analyses approved the method of in situ PCR to be highly reliable. The factors which could affect the chemiluminiscence were studied in detail. The results showed that the MNPs of 400 nm in diameter are beneficial to the detection. The sequence length and the binding site of the probe with a target sequence have obvious effects on the detection. The optimal concentration of the probes, hybridization temperature and hybridization time were 10 muM, 60 masculineC and 60 mins, respectively. The method of in situ PCR based on MNPs can greatly improve the utilization rate of the DNA template ultimately enhancing the detection sensitivity. Experiment results proved that the primer and probe had high specificity, and Pseudomonas aeruginosa was successfully detected with detection limits as low as 10 cfu/mL by this method, while the detection of a single Pseudomonas aeruginosa can also be achieved.
摘要：
Biodegradable core/shell structured poly(L-lactide) acid (PLLA)/chitosan (CS) nanofibers were fabricated by coaxial electrospinning. PLLA and CS were dissolved in dichloromethane and aqueous acetic acid solvents for spinning into core and shell layers, respectively. CS of high molecular weight was difficult to spin into nanofibers by electrospinning due to its high viscosity, but it was easier to achieve by coaxial electrospinning with PLLA. The preparation conditions were optimized by changing the ratios of PLLA/CS under different jet voltages. After being investigated by scanning electron microscope (SEM), a smooth structure was prepared using 2% CS as the shell solution with applied voltage 15 kV. Transmission electron microscopy (TEM) study and infrared spectrometry (IR) characterization of PLLA/CS nanofibers indicated that the core/shell structure was successfully fabricated. Brunauer-Emmett-Teller (BET) surface area and pore size distribution exhibited higher capacity of PLLA/CS than PLLA used as drug carrier in tissue engineering. The cytocompatibility of nanofibers were evaluated by co-cultured with human bone marrow-derived UE7T-13 cells, the 3-(4,5)-dimethylthiahiazo (-z-y1)-3,5-di-phenytetrazoliumromide (MTT) test exhibited good proliferation of PLLA/CS for cells. Results of blood compatibility tests showed decreased hemolytic ratio and platelets adhesion of PLLA/CS compared with PLLA. The results indicated that PLLA/CS nanofibers could be potential drug carrier for tissue engineering.
作者：
Mei Liu;Xin Zeng;Chao Ma;Huan Yi;Zeeshan Ali;Xianbo Mou;Song Li;Yan Deng;Nongyue He
期刊：
Bone Research,2017年5(2):75-94 ISSN：2095-4700
通讯作者：
Deng, Yan;He, Nongyue
作者机构：
[Xianbo Mou; Chao Ma; Yan Deng; Nongyue He; Deng, Yan; He, Nongyue; Mei Liu; Huan Yi] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing, Jiangsu, Peoples R China.;[Xin Zeng] Nanjing Matern & Child Hlth Care Hosp, Nanjing, Jiangsu, Peoples R China.;[Zeeshan Ali] Shenzhen Polytech, Sch Appl Chem & Biotechnol, Shenzhen, Peoples R China.;[Zeeshan Ali] Southeast Univ, Sch Chem & Chem Engn, Nanjing, Jiangsu, Peoples R China.;[Song Li; Yan Deng; Nongyue He; Deng, Yan; He, Nongyue] Hunan Univ Technol, Hunan Key Lab Green Chem & Applicat Biol Nanotech, Zhuzhou, Peoples R China.
通讯机构：
[Deng, Y; He, NY] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing, Jiangsu, Peoples R China.;[Deng, Y; He, NY] Hunan Univ Technol, Hunan Key Lab Green Chem & Applicat Biol Nanotech, Zhuzhou, Peoples R China.
关键词：
HYDROGELS;CARTILAGE;ENGINEERING
摘要：
Tissue engineering has become a promising strategy for repairing damaged cartilage and bone tissue. Among the scaffolds for tissue-engineering applications, injectable hydrogels have demonstrated great potential for use as three-dimensional cell culture scaffolds in cartilage and bone tissue engineering, owing to their high water content, similarity to the natural extracellular matrix (ECM), porous framework for cell transplantation and proliferation, minimal invasive properties, and ability to match irregular defects. In this review, we describe the selection of appropriate biomaterials and fabrication methods to prepare novel injectable hydrogels for cartilage and bone tissue engineering. In addition, the biology of cartilage and the bony ECM is also summarized. Finally, future perspectives for injectable hydrogels in cartilage and bone tissue engineering are discussed.
摘要：
A sensitive and selective biosensor platform suited for SNP type using Fe@Au magnetic nanoparticles (GMNPs) to fabricate bead array is described. This new platform integrates the rapid binding kinetics of magnetic nanoparticles carriers, the multiplexing and encoding capabilities of chips, and tagged array. As a DNA sensor, the biotinylated single-stranded DNA was obtained by asymmetry PCR amplification, and then captured by GMNPs modified with streptavidin to form GMNP-ssDNA complexes without further purification. The complexes were immobilized on the slide to fabricate bead array through magnetic field. The bead array was hybridized with the corresponding allele-specific tag probes for each locus, and a pair of given universal detectors were applied to these markers analysis. Using bead array, all samples can be analyzed in one hybridization chamber which lowers the cost of the assay. Using universal tags, only a pair of universal dual-color probes labeled fluorophores was used for multiplex genotyping. Without the need of laborious and time-consuming elution, the experiment process was simple, reproducible and easy to handle. Two SNPs loci from 12 individual samples were discriminated using this platform and the results demonstrated that the expected scores and good discrimination were obtained between the two alleles from the two SNP loci. In summary, the integrated sensitive platform is adaptable and versatile, while offering a high-throughput capability needed for genome research and clinical applications. (C) 2010 Elsevier B.V. All rights reserved.
关键词：
heavy metal ions;sensors;nanomaterials;detection;progress
摘要：
The heavy metal ions, especially Cd~(2+), Pb~(2+) and Hg~(2+), show extremely hazard to the environment and human being. The measurement of heavy metal ions using sensors is catching more and more attention for its advantages of high sensitivity and selectivity, low-cost, convenience to handle and rapid detection. In recent years, nanomaterials such as gold nanoparticles (NPs), magnetic nanoparticles, graphene and nanocomposite materials are applied in sensors for improving sensitivity and selectivity, making the research on electrochemical (EC) sensors, spectrometric biosensors and colorimetric biosensors become a hot spot in the application to investigate heavy metal ions, in particular, Cd~(2+), Pb~(2+) and Hg~(2+). In this short review, the research of advanced detection of Cd~(2+), Pb~(2+) and Hg~(2+) and its progress based on nanomaterial sensors in recent years is reviewed.
期刊：
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2013年9(4):674-684(11) ISSN：1550-7033
通讯作者：
He, N.(nyhe1958@163.com)
作者机构：
[Jin, Lian; Liu, Ming; Xi, Zhijiang; Deng, Yan; Li, Chuanyan; Jiang, Hongrong; Li, Zhiyang; He, Nongyue] Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China.;[Zeng, Xin] Nanjing Med Univ, State Key Lab Reprod Med, Dept Gynecol Endocrine, Nanjing Matern & Child Hlth Care Hosp, Nanjing 210029, Jiangsu, Peoples R China.;[Wang, Zhifei] Southeast Univ, Sch Chem & Chem Engn, Nanjing 211189, Jiangsu, Peoples R China.;[Deng, Yan; He, Nongyue] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412007, Peoples R China.
通讯机构：
[He, Nongyue] Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
CHARACTERIZATIONS;FLUORESCENCE;MULTI-STEP CHEMICAL REDUCTION;SEED-MEDIATED GROWING;SELF-ASSEMBLING;[email protected]@AU MAGNETIC NANOCOMPOSITES
摘要：
In present study, we put forward an approach to prepare three-layer core shell Fe3O4@SiO2@Au magnetic nanocomposites via the combination of self-assembling, seed-mediated growing and multi-step chemical reduction. The Fe3O4@SiO2@Au magnetic nanoconnposites were analyzed and characterized by transmission electron microscope (TEM), scanning electronic microscope (SEM), energy dispersive spectrometer analysis (EDS), Fourier transform infrared spectroscopy (FT-IR), vibrating sample magnetometer (VSM), and ultraviolet and visible spectrophotometer (UV-Vis). TEM and SEM characterizations showed that the Fe3O4@SiO2@Au nanocomposites were obtained successfully with three-layer structures, especially a layer of thin, smooth and continuous gold shell. The average diameter of Fe3O4@SiO2@Au nanocomposites was about 600 nm and an excellent dispersity was observed for the as-prepared nanoparticles. EDS characterizations demonstrated that the nanocomposites contained three elements of the precursors, Fe, Si, and Au. Furthermore, FT-IR showed that the silica and gold shell were coated successfully. UV-Vis and VSM characterizations showed that the Fe3O4@SiO2@Au nanocomposites exhibited good optical and magnetic property, and the saturation magnetization was 25.76 emu/g. In conclusion, the Fe3O4@SiO2@Au magnetic nanoconnposites with three-layer core shell structures were prepared. Furthermore, Fe3O4@SiO2@Au magnetic nanocomposites were modified with streptavidin (SA) successfully, and it was validated that they performed low fluorescence background, suggesting that they should have good applications especially in bioassay based on fluorescence detection through bonding the biotinylated fluorescent probes.
作者机构：
[Jin, Lian; Li, Song; Liu, Ming; Elingarami, Sauli; Deng, Yan; Zhang, Gen; Liu, Hongna; Fan, Jing; Zeng, Xin; Yang, Haowen; He, Nongyue] Southeast Univ, Dept Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.;[Zeng, Yu; Zeng, Xin; Hu, Ping] Nanjing Matern & Child Hlth Care Hosp, Nanjing 210029, Jiangsu, Peoples R China.;[Zhang, Gen] Nanjing Med Univ, Nanjing 210029, Jiangsu, Peoples R China.;[Li, Song; Deng, Yan] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412007, Peoples R China.
通讯机构：
[Zeng, Xin] Southeast Univ, Dept Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
Copy number variation;Ligation-dependent PCR;Magnetic nanoparticles;Chemiluminescence
摘要：
A novel system for copy number variation (CNV) analysis was developed in the present study using a combination of magnetic separation and chemiluminescence (CL) detection technique. The amino-modified probes were firstly immobilized onto carboxylated magnetic nanoparticles (MNPs) and then hybridized with biotin-dUTP products, followed by amplification with ligation-dependent polymerase chain reaction (PCR). After streptavidin-modified alkaline phosphatase (STV-AP) bonding and magnetic separation, the CL signals were then detected. Results showed that the quantification of PCR products could be reflected by CL signal values. Under optimum conditions, the CL system was characterized for quantitative analysis and the CL intensity exhibited a linear correlation with logarithm of the target concentration. To validate the methodology, copy numbers of six genes from the human genome were detected. To compare the detection accuracy, multiplex ligation-dependent probe amplification (MLPA) and MNPs-CL detection were performed. Overall, there were two discrepancies by MLPA analysis, while only one by MNPs-CL detection. This research demonstrated that the novel MNPs-CL system is a useful analytical tool which shows simple, sensitive, and specific characters which are suitable for CNV analysis. Moreover, this system should be improved further and its application in the genome variation detection of various diseases is currently under further investigation.
作者：
Yi Huan;Rehman Fawad Ur;Zhao Chunqiu;Liu Bin;He Nongyue
期刊：
Bone Research,2016年4(4):206-216 ISSN：2095-4700
通讯作者：
He, Nongyue
作者机构：
[Ur Rehman, Fawad; Zhao Chunqiu; Yi Huan] State Key Laboratory of Bioelectronics (Chien-Shiung Wu Lab), School of Biological Science and Medical Engineering, Southeast University , Nanjing, China;[Liu Bin] Department of Biomedical Engineering, School of Basic Medical Sciences, Nanjing Medical University , Nanjing, China;[He Nongyue] State Key Laboratory of Bioelectronics (Chien-Shiung Wu Lab), School of Biological Science and Medical Engineering, Southeast University, Nanjing, China;[He Nongyue] Hunan Key Laboratory of Green Chemistry and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, China
通讯机构：
[He, Nongyue] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Chien Shiung Wu Lab, Nanjing, Jiangsu, Peoples R China.;[He, Nongyue] Hunan Univ Technol, Hunan Key Lab Green Chem & Applicat Biol Nanotech, Zhuzhou, Peoples R China.
关键词：
医学;骨科学
摘要：
Biomedical applications of nanomaterials are exponentially increasing every year due to analogy to various cell receptors, ligands, structural proteins, and genetic materials (that is, DNA). In bone tissue, nanoscale materials can provide scaffold for excellent tissue repair via mechanical stimulation, releasing of various loaded drugs and mediators, 3D scaffold for cell growth and differentiation of bone marrow stem cells to osteocytes. This review will therefore highlight recent advancements on tissue and nanoscale materials interaction.
作者：
Jiang, Hongrong;Zeng, Xin;He, Nongyue;Deng, Yan;Lu, Guangming;Li, Kai
期刊：
Journal of Nanoscience and Nanotechnology,2013年13(3):1617-1625 ISSN：1533-4880
通讯作者：
He, N.
作者机构：
[He, Nongyue; Deng, Yan; Jiang, Hongrong] State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China;[Li, Kai] Laboratory of Molecular Medicine, College of Pharmaceutical Science, Soochow University, Suzhou 215123, China;[Lu, Guangming] Department of Radiology, Nanjing University, Jin Ling Hospital of Nanjing, Nanjing 210002, China;[Deng, Yan] Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China;[Zeng, Xin] State Key Laboratory of Reproductive Medicine, Department of Gynecological Endocrine, Nanjing Maternity, Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing 210029, China
通讯机构：
[He, Nongyue] Southeast Univ, Sch Biol Sci & Med Engn, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China.
关键词：
Gold-Coated Magnetic Nanocomposites;Preparation;Approaches;Biomedical Applications
摘要：
Gold-coated magnetic nanocomposites with unique physical and chemical properties have recently been subjected to extensive researches since the gold shells could improve their biocompatibility, functionality and stability further, which endue them with great application potentials in various fields. Several main approaches have been studied for the synthesis of gold-coated magnetic nanocomposites, including microemulsion, sonochemical synthesis, self assembly, seed-mediated growth method, and laser irradiation. This review is focused on describing various strategies for the preparation of gold-coated magnetic nanocomposites. Furthermore, we also introduce main applications of gold-coated magnetic nanocomposites in biomedical fields and point out some challenges in the synthesis of high-quality gold-coated magnetic nanocomposites.
摘要：
A novel acetylcholinesterase (AChE) biosensor was developed based on layer-by-layer assembly of chitosan (CS), gold nanoparticles (GNPs), AChE and Nafion (NF) modified nano-porous pseudo carbon paste electrode (nano-PPCPE). The proposed biosensor exhibited a high affinity to the substrate of AChE and produced a detectable and fast response owing to its excellent electron transfer rate and satisfactory biocompatibility of GNPs/CS. Nano-PPCPE displayed good electrode chemical behavior, low detection limit and good stability. Nafion (NF) was used as a protective membrane of the AChE biosensor. The AChE biosensor showed favorable affinity to acetylthiocholine iodide (ATChI) with wide linear range (1.0 x 10(-4)-1.0 x 10(-7) M) and an ultralow detection limit of 1.0 x 10(-8) M. Under optimum conditions, the biosensor detected methyl parathion in the linear range from 1.0 x 10(-8) to 1.0 x 10(-14) g/mL with a detection limit of 5,0 x 10(-15) g/mL. The proposed biosensor exhibited good sensitivity, stability, and reproducibility, thus providing a promising analytical tool for pesticide detection.
