[Deng, Yan; Dong, Hongming; Liu, Yuan; He, Nongyue; Liu, Keke] Economical Forest Cultivation and Utilization of 2011 Collaborative Innovation Center in Hunan Province, Hunan Key Laboratory of Green Chemistry and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou, 412007, China;[He, Nongyue] State Key Laboratory of Bioelectronics, Southeast University, Nanjing, 210096, China
heavy metal ions;sensors;nanomaterials;detection;progress
The heavy metal ions, especially Cd~(2+), Pb~(2+) and Hg~(2+), show extremely hazard to the environment and human being. The measurement of heavy metal ions using sensors is catching more and more attention for its advantages of high sensitivity and selectivity, low-cost, convenience to handle and rapid detection. In recent years, nanomaterials such as gold nanoparticles (NPs), magnetic nanoparticles, graphene and nanocomposite materials are applied in sensors for improving sensitivity and selectivity, making the research on electrochemical (EC) sensors, spectrometric biosensors and colorimetric biosensors become a hot spot in the application to investigate heavy metal ions, in particular, Cd~(2+), Pb~(2+) and Hg~(2+). In this short review, the research of advanced detection of Cd~(2+), Pb~(2+) and Hg~(2+) and its progress based on nanomaterial sensors in recent years is reviewed.
Lai, Yuxuan;Wang, Lijun;Liu, Yuan;Yang, Gaojian;Tang, Congli;Deng, Yan;Li, Song
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY,2018年14(1):44-65 ISSN：1550-7033
[Yang, Gaojian; Lai, Yuxuan; Liu, Yuan; Li, Song; Deng, Yan; Tang, Congli] Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou, 412007, China;[Wang, Lijun] Guangxi Key Laboratory of Clean Pulp and Paper Making and Pollution Control, Nanning, 530007, China;[Wang, Lijun] Collaborative Innovation Center for Guangxi Sugar Industry, Guangxi, Nanning, 530004, China;[Wang, Lijun] College of Light Industry and Food Engineering, Guangxi University, Nanning, 530004, China
A novel molecular imprinted polymer (MIP) electrochemical sensor was successfully fabricated for sensitive detection of carcinomaembryonic antigen (CEA). We used CEA as template, dopamine (DA) as imprinted monomers. Through controlling electropolymerization, a "PDA-CEA" complex was achieved. After elution, the specific cavities adsorbed the target molecules. In addition, polythionine (PTh) and AuNPs were applied as the electrode modifying materials to enhance electron transfer rate and improve detection signal. Using differential pulse voltammetry (DPV) detection, the peak current decreased with the increase in concentration of CEA, and the linear response range of the MIP sensor was from 0.001 ng/mL to 1000 ng/mL with the detection limit as low as 0.2589 pg/mL. The MIP sensor had a low sample consumption, good stability, and high sensitivity, and could become a new promising method for the detection of CEA. Furthermore, this MIP sensor was demonstrated in testing CEA in human serum sample with satisfactory results.
A mercury ion aptamer electrochemical biosensor based on a Thymine-Hg2+-Thymine (T-Hg2+-T) structure has been constructed and successfully used to detect mercury ions in tap water samples. The aptamer electrochemical biosensor was assembled using a mercury ion aptamer-functionalized AuNPs-modified glass carbon electrode (aptamer/(AuNPs/CS)(2)/GCE) capable of specifically detecting mercury ions through a T-Hg2+-T structure. The experimental results indicated the optimum electrochemical performance of the prepared aptamer biosensor when the (AuNPs/CS)(2)/GCE surface was modified with 1.0 mu M aptamer and incubated with Hg2+ for 60 min. Moreover, the aptamer biosensor exhibits a good linear relationship between the logarithm of the Hg2+ concentration and the DPV peak current in the range from 0.01 to 500 nM following the linearization equation I-p (mu A) = 2.59902 +0.2097 logC (R-2 = 0.9994) with a limit of detection as low as 0.005 nM. Therefore, the constructed aptamer biosensor provides a simple and sensitive approach for Hg2+ detection in aqueous solution with promising application for trace Hg2+ detection in real samples.