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Preparation and electrochemical behavior of L-glutamate electrochemical biosensor.

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WOS被引频次:205
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成果类型:
期刊论文
作者:
Deng, Yan;Wang, Wei;Zhang, Liming;Lu, Zhuoxuan;Li, Song;Xu, Lijian
通讯作者:
Deng, Y
作者机构:
[Lu, Zhuoxuan; Zhang, Liming; Deng, Yan] State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
[Wang, Wei; Li, Song; Xu, Lijian; Lu, Zhuoxuan; Zhang, Liming; Deng, Yan] Hunan Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China
通讯机构:
[Deng, Yan] Hunan Univ Technol, Hunan Key Lab Green Packaging & Applicat Biol Nan, Zhuzhou 412007, Peoples R China.
语种:
英文
关键词:
Bovine serum albumins - Carbon paste electrode - Electrochemical - Electrochemical behaviors - Electrochemical biosensor - L-glutamate - L-Glutamate Oxidase - Phosphate buffers
期刊:
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
ISSN:
1550-7033
年:
2013
卷:
9
期:
2
页码:
318-321
文献类别:
WOS:Article;EI:Journal article (JA)
所属学科:
ESI学科类别:生物学与生物化学;WOS学科类别:Medicine, Research & Experimental;Nanoscience & Nanotechnology
入藏号:
WOS:000314192000023;EI:20131816285560;PMID:23627063
基金类别:
Chinese 973 Project [2010CB933903]; Natural Science Foundation of China [60971045, 60927001, 61001053]; Hunan Science and Technology Projects [2012SK3105, 2010sk2003]; Natural Science Foundation of Hunan Province, China [11JJ5011]; Research Foundation of Education Bureau of Hunan Province, China [11A030]; State Key Laboratory of Electronics, China [2011E27]
机构署名:
本校为第一且通讯机构
院系归属:
包装与材料工程学院
摘要:
Using the nano-porous pseudo carbon paste electrode (Nano-PPCPE) as the working electrode, mixing L-glutamate oxidase (L-GLOD), catalase (Cat) and bovine serum albumin (BSA) with phosphate buffer (PB, pH = 7.4), followed by cross-linking with glutaraldehyde, a novel L-glutamate electrochemical biosensor was successfully formed. It was demonstrated that the modified nano- PPCPE exhibits a high selectivity and sensitivity in comparison with the modified CPE. The L-glutamate biosensor showed a linear range from 5&times;10<sup>-7</sup> M to 1&times;10<sup>-5</sup> M with the detection limit of 2.5&times;10 <sup>-7</sup> M. Copyright &copy;2013 American Scientific Publishers All rights reserved.
参考文献:
Basu Anjan Kumar, 2006, Indian Journal of Experimental Biology, V44, P392
Bitar A, 2012, J BIOMED NANOTECHNOL, V8, P709, DOI 10.1166/jbn.2012.1439
Brown JT, 2007, EUR J NEUROSCI, V25, P2982, DOI 10.1111/j.1460-9568.2007.05544.x
Cao JL, 2012, J BIOMED NANOTECHNOL, V8, P770, DOI 10.1166/jbn.2012.1447
Choudhari K, 2011, J BIOMED NANOTECHNOL, V7, P70, DOI 10.1166/jbn.2011.1206

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