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An integrated and sensitive detection platform for biosensing application based on Fe@Au magnetic nanoparticles as bead array carries

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WOS被引频次:165
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成果类型:
期刊论文、会议论文
作者:
Liu, Hongna;Li, Song;Liu, Lishang;Tian, Lan;He, Nongyue
通讯作者:
He, N.(nongyue580210@yahoo.com.cn)
作者机构:
[Tian, Lan; Li, Song; He, Nongyue] Key Lab. of Green Packaging and Application of Biological Nanotechnology of Hunan Province, Hunan University of Technology, Zhuzhou 412008, China
[Li, Song; He, Nongyue; Liu, Hongna; Liu, Lishang] State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
通讯机构:
[He, Nongyue] Southeast Univ, State Key Lab Bioelect, Sch Biol Sci & Med Engn, Nanjing 210096, Peoples R China.
语种:
英文
关键词:
Bead array;Universal tags;Dual-color hybridization;Single nucleotide polymorphisms
期刊:
Biosensors and Bioelectronics
ISSN:
0956-5663
年:
2010
卷:
26
期:
4
页码:
1442-1448
文献类别:
WOS:Article;Proceedings Paper;EI:Journal article (JA)
所属学科:
ESI学科类别:化学;WOS学科类别:Biophysics;Biotechnology & Applied Microbiology;Chemistry, Analytical;Electrochemistry;Nanoscience & Nanotechnology
入藏号:
WOS:000286403400045;EI:20104913466839;PMID:20728338
机构署名:
本校为其他机构
院系归属:
包装与材料工程学院
摘要:
A sensitive and selective biosensor platform suited for SNP type using Fe@Au magnetic nanoparticles (GMNPs) to fabricate bead array is described. This new platform integrates the rapid binding kinetics of magnetic nanoparticles carriers, the multiplexing and encoding capabilities of chips, and tagged array. As a DNA sensor, the biotinylated single-stranded DNA was obtained by asymmetry PCR amplification, and then captured by GMNPs modified with streptavidin to form GMNP-ssDNA complexes without further purification. The complexes were immobilized on the slide to fabricate bead array through magnetic field. The bead array was hybridized with the corresponding allele-specific tag probes for each locus, and a pair of given universal detectors were applied to these markers analysis. Using bead array, all samples can be analyzed in one hybridization chamber which lowers the cost of the assay. Using universal tags, only a pair of universal dual-color probes labeled fluorophores was used for multiplex genotyping. Without the need of laborious and time-consuming elution, the experiment process was simple, reproducible and easy to handle. Two SNPs loci from 12 individual samples were discriminated using this platform and the results demonstrated that the expected scores and good discrimination were obtained between the two alleles from the two SNP loci. In summary, the integrated sensitive platform is adaptable and versatile, while offering a high-throughput capability needed for genome research and clinical applications. (C) 2010 Elsevier B.V. All rights reserved.
参考文献:
Akutsu J, 2004, BIOTECHNOL BIOENG, V86, P667, DOI 10.1002/bit.20049
Fan JB, 2006, NAT REV GENET, V7, P632, DOI 10.1038/nrg1901
Gharizadeh B, 2003, NUCLEIC ACIDS RES, V31, DOI 10.1093/nar/gng147
Gresham D, 2006, SCIENCE, V311, P1932, DOI 10.1126/science.1123726
Katz E, 2004, ANGEW CHEM INT EDIT, V43, P6042, DOI 10.1002/anie.200400651

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