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A novel acetylcholine bioensor and its electrochemical behavior.

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WOS被引频次:188
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成果类型:
期刊论文
作者:
Wang, Wei;Deng, Yan;Li, Song;Liu, Hongna;Lu, Zhuoxuan;Zhang, Liming;Lin, Lin;Xu, Lijian
通讯作者:
Deng, Y
作者机构:
[Deng, Yan; Liu, Hongna] State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
[Zhang, Liming; Lu, Zhuoxuan; Wang, Wei; Xu, Lijian; Li, Song; Deng, Yan; Lin, Lin] Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China
通讯机构:
[Deng, Y] Hunan Univ Technol, Key Lab Green Packaging & Applicat Biol Nanotechn, Zhuzhou 412007, Peoples R China.
语种:
英文
关键词:
Acetylcholinesterase;Acetylthiocholine Iodide;Electrochemical;Nano-Porous Pseudo Carbon Paste Electrode
期刊:
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
ISSN:
1550-7033
年:
2013
卷:
9
期:
4
页码:
736-740
文献类别:
WOS:Article;EI:Journal article (JA)
所属学科:
ESI学科类别:生物学与生物化学;WOS学科类别:Materials Science, Biomaterials;Nanoscience & Nanotechnology
入藏号:
WOS:000323915600025;EI:20131816285610;PMID:23621036
基金类别:
National Key Program for Developing Basic Research of China [2010CB933903]; NSFC [60971045, 21205013, 21205036, 61271056, 61001053]; Hunan Science and Technology Projects [2012SK3105, 2010sk2003]; Natural Science Foundation of Hunan Province [12JJ6060, 12JJ9038]; Scientific Research Fund of Hunan Provincial Education Department [11A030]; China Postdoctoral Science Foundation [2012M520980, 2012M520981]
机构署名:
本校为第一且通讯机构
院系归属:
包装与材料工程学院
摘要:
A novel type of acetylcholine electrochemical biosensor was successfully fabricated by dropping &beta;-cyclodextrin solution, acetylcholinesterase solution and Nafion-methanol solution to the homemade nano-porous pseudo carbon paste electrode (nano-PPCPE) as working electrode, and then the electrochemical behavior of the as-prepared biosensor was studied through the acetylthiocholine iodide (ATChI). It was shown that the modified nano-PPCPE displays a high selectivity and sensitivity. The acetylcholine biosensor showed the linear range from 5&times;10<sup>-6</sup> M to 2&times;10<sup>-4</sup> M with the detection limit of 1.5&times;10<sup>-7</sup> M. Copyright &copy;2013 American Scientific Publishers All rights reserved.
参考文献:
Arduini F, 2012, ELECTROANAL, V24, P581, DOI 10.1002/elan.201100540
Carvajal A. M. F., 2006, J MOL NEUROSCI, V30, P121
Chan SHS, 2012, PEDIATR NEUROL, V47, P137, DOI 10.1016/j.pediatrneurol.2012.04.022
Ebrahimi B., 2012, BIOTECHNOL BIOPROC E, V15, P383
Evtugyn GA, 2012, ELECTROANAL, V24, P554, DOI 10.1002/elan.201100538

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