Fabrication of an electrochemical biosensor array for simultaneous detection of L-glutamate and acetylcholine.

成果类型：

期刊论文

作者：

Deng, Yan;Wang, Wei;Ma, Chao;Li, Zhiyang

通讯作者：

Deng, Y.(dy_dengyan@yahoo.com.cn)

作者机构：

[Li, Zhiyang; Ma, Chao; Deng, Yan] State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China

[Wang, Wei; Deng, Yan] Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China

通讯机构：

[Deng, Yan] Key Laboratory of Green Packaging and Application of Biological Nanotechnology, Hunan University of Technology, Zhuzhou 412007, China.

语种：

英文

关键词：

Glutamate;Acetylcholinesterase (AChE);Electrochemical Biosensor;Nano-Porous Pseudo Carbon Paste Electrode (Nano-PPCPE);Neurotransmitter

期刊：

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY

ISSN：

1550-7033

年：

2013

卷：

9

期：

8

页码：

1378-1382

文献类别：

WOS:Article;EI:Journal article (JA)

所属学科：

ESI学科类别:生物学与生物化学;WOS学科类别:Materials Science, Biomaterials;Nanoscience & Nanotechnology

入藏号：

WOS:000322605500009;EI:20133216577802;PMID:23926804

DOI：

10.1166/jbn.2013.1633

基金类别：

National Key Program for Developing Basic Research of China [2010CB933903]; NSFC [60971045, 21205013, 21205036, 61271056, 61201033]; Hunan Science and Technology Projects [2012SK3105, 2010sk2003]; Natural Science Foundation of Hunan Province [12JJ6060, 12JJ9038]; Scientific Research Fund of Hunan Provincial Education Department [11A030]; China Postdoctoral Science Foundation [2012M520980]

机构署名：

本校为第一且通讯机构

院系归属：

包装与材料工程学院

摘要：

Using two nano-porous pseudo carbon paste electrodes (Nano-PPCPEs) as the working electrodes, a saturated calomel electrode as the reference electrode, and a Pt electrode as the counter electrode to form a four-electrode system, a novel type of glutamate-acetylcholine electrochemical biosensor array was successfully fabricated. It was shown that the biosensor array displayed a high selectivity and sensitivity and the selective and simultaneous determination of glutamate and acetylcholine (ATCh) is feasible. For L-glutamate, a linear range from 5×10-7 M to 1×10-5 M with the detection limit of 2.5×10-7 M was demonstrated, while for ATChI the linear range is from 5×10-6 M to 2×10-4 M with the detection limit of 1.5×10-7 M. This research means a possibility to simultaneously detect more neurotransmitters with a nano-PPCPEs electrochemical biosensor array. Copyright ©2013 American Scientific Publishers All rights reserved.

参考文献：

Ali Z, 2012, J NANOSCI NANOTECHNO, V12, P8241, DOI 10.1166/jnn.2012.6692

Arduini F, 2012, ELECTROANAL, V24, P581, DOI 10.1002/elan.201100540

Bazaka K, 2012, MATER EXPRESS, V2, P285, DOI 10.1166/mex.2012.1086

Chan SHS, 2012, PEDIATR NEUROL, V47, P137, DOI 10.1016/j.pediatrneurol.2012.04.022

Deng Y, 2013, J BIOMED NANOTECHNOL, V9, P318, DOI 10.1166/jbn.2013.1487

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